Selection of Producer of α-Ketoglutaric Acid from Ethanol-Containing Wastes and Impact of Cultivation Conditions

Ester–aldehyde fraction (EAF) is a by-product of ethyl-alcohol-producing companies whose purification requires an expensive process. The results this study illustrate the environmentally friendly and alternative possibility using EAF to increase their value as substrate produce α-ketoglutaric acid (KGA) different yeasts. It was found that some species genera Babjeviella, Diutina, Moesziomyces, Pichia, Saturnispora, Sugiyamaella, Yarrowia Zygoascus grown under thiamine deficiency accumulate KGA in medium with sole carbon source. strain Y. lipolytica VKM Y-2412 selected producer. To reach maximum production KGA, cultivation should contain 0.3 µg/L during flasks 2 fermentor; concentration (NH4)2SO4 range from 3 6 g/L; optimal concentrations Zn2+, Fe2+ Cu2+ ions be 1.2, 0.6 0.05 mg/L, respectively. not exceed 1.5 g/L growth phase synthesis phase. At higher concentrations, acetic accumulated inhibited yeast production. Under conditions, producer 53.8 yield (Yp/s) 0.68 g/g consumed.